Team:BostonU/Data

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BostonU iGEM Team: Welcome


Data Collected


    Sequencing Data for Converted MoClo Parts


    Characterizing BioBrick Based Devices









    Characterizing MoClo and BioBrick Based Devices



    Characterizing New BioBrick Parts
      A New Repressible System: Copper and mmoR

        We are also introducing a new promoter and gene pair to iGEM that forms a copper-dependent repressible system. Since the library of metal sensitive promoters was relatively small on the Registry of Standard Biological Parts, we decided to investigate a metal-dependent system. We chose mmoR and a σ54 promoter associated with it from a bacterium called Methylosinus trichosporium OB3b. mmoR is a σ54-dependent transcriptional activator.

        It is unclear if the σ54 promoter will function in E. coli in the absence of mmoR. BLAST results comparing the mmoR to the E. coli genomes available yielded very short hits, with the longest matching



        Scanlan et. al, 2009

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