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• Results
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  • Datapage
  • Laccases
  • Immobilization
  • Substrate Analysis
  • Cellulose Binding Domain
  • Shuttle Vector
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Our BioBricks

<groupparts>iGEM012 Bielefeld-Germany</groupparts>

Datapage

How our BioBrick works

Figure 1: iGEM Team Bielefeld is developing a biological filter using immobilized laccases, enzymes able to radicalize and break down a broad range of aromatic substances. For the production of laccases from different bacteria, fungi and plants, two expression systems are used: Escherichia coli and the yeast Pichia pastoris. Immobilization is carried out either by using CPC-silica beads or by fusing the enzymes to cellulose binding domains. The concept could be extended to other toxic pollutants in drinking and wastewater, as well as to industrial applications in paper and textile industries or even for bioremediation of contaminated soil.

Data for our favorite new parts

1. <partinfo>K863000</partinfo> - bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and His-tag: This part is used to overexpress the laccase bpul for further purification followed by characterization of enzyme activity and substrate specificity.
2. <partinfo>K863005</partinfo> - ecol (laccase from E. coli) with T7 promoter, RBS and His-tag: This enzyme is overexpressed after induction and can be purified by His-tag. Subsequently the laccase can be characterized regarding to enzyme activity and substrate specificity.
3. <partinfo>K863204</partinfo> - shuttle vector pECPP11JS for site-directed recombination of genes of interest in yeast: With this part the production and secretion of a protein of interest like laccase is possible.

We have also characterized the following parts

1. <partinfo>BBa_K863012</partinfo> - tthl laccase (from T. thermophilus) with constitutive promoter J23100, RBS and His-tag: This part is used for the constitutive expression of tthl for further purification followed by characterization of enzyme activity and substrate specificity.
2. <partinfo>BBa_K863022</partinfo> - bhal laccase (from Bacillus halodurans) with constitutive promoter J23100, RBS and His-tag: This part is used for the constitutive expression of bhal for further purification followed by characterization of enzyme activity and substrate specificity.

Judging criteria

Bronze criteria

Registration of our team

Judging form completed

Team Wiki constructed

Presentation of a poster and a talk at the iGEM Jamboree

Either one new submitted and highly-documented standard BioBrick Part or Device, or a new application of and outstanding documentation of an already existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry.

Silver criteria

In addition to the Bronze criteria...

• Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected
• Characterization of the operation of at least one new BioBrick Part/ Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry.

Gold criteria

One or more of the following aspects in addition to the Bronze & Silver criteria...

• Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year) and enter this information in the Registry (in the “Experience” section of that BioBrick’s Registry entry), and don't forget to create a new registry page for the improved part.
• Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.
• Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.

Collaboration