Team:Valencia/protocols

From 2012.igem.org

Revision as of 01:22, 27 September 2012 by Daie07 (Talk | contribs)



Protocols


Culture medium

  • LB Agar Plates
  • BG-11 for Wildtype Liquid
  • BG-11 for Wildtype Solid
  • BG-11 for Harvard/Golden Liquid
  • BG-11 for Harvard/Golden Solid
  • Compo Plus Agar


Culture

  • Culturing E.Coli
  • Culturing S. elongatus in liquid medium
  • Culturing S. elongatus in solid medium


Molecular Tools

  • Transforming Chemically Competent Cells E.Coli DH5α-T1R
  • PLASMID MINIPREP
  • DNA Digestion
  • Ligations
  • Agarose Gel Electrophoresis
  • Transforming: S. elongatus PCC7942