Team:Paris Bettencourt/Overview

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Delay system: This module will permit our bacteria to perform its function during X amount of time before triggering the degradation and suicide of the bacteria. The circuit of this

Restriction Enzyme system system.

The suicide system is a variation of a toxin-anti-toxin system, and consists of a lethal DNA cleaving protein which is bound with high affinity by the anti-toxin immunity protein. The anti-toxin plasmid carrying the immunity protein, when combined with the restriction enzyme system, is degraded on a tunable delay. Once the anti-toxin plasmid degrades, the toxin kills the cell, its neighbors, and eliminates extracellular DNA via its DNase activity.

Toxin/anti-Toxin system.

Toxin/anti-Toxin system.

Objectives

Our project aims to:

• Raise the issue of biosafety, and advocate the discerning use of biosafety circuits in future iGEM projects as a requirement
• Evaluate the risk of HGT in different SynBio applications
• Develop a new, improved containment system to expand the range of environments where GEOs can be used safely.

To do so, we:

• Engaged the general public and scientific community through debate
• Raised the question about how we can regulate this practices
• Compiled a parts page of safety circuits in the registry
• Relied on three levels of containment :
  1. Physical containment with alginate capsules
  2. Semantic containment using an amber suppressor system
  3. An improved killswitch featuring delayed population-level suicide through complete genome degradation.

We strived to make our system as robust against mutations as possible.

Step 1

Step 2

Step 2.

Step 3

Step 3.