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Contents 1 Computational Data 1.1 Maps 1.1.1 Tar_QC 1.1.2 flhDC 1.1.3 fliC 1.1.4 motA 1.1.5 motB 1.1.6 yhjH 1.1.7 Constitutive promoter + RFP-constructs 1.2 Sequences 1.2.1 Primers 1.3 Raw Datasets 1.3.1 Characterization of constitutive promoters by qrtPCR

Computational Data

Maps

Tar_QC

For Parts K777000 - K777008 the plasmid maps were constructed in ApE. Within the maps you will find annotated features, as well. To gain more information about the parts submitted by the iGEM team Göttingen 2012 click here.
Moreover, the parts were completely sequenced (except for K777000) and aligned. The original data and the reviewed data are provided in the respective Zip- files.

• ApE plasmid maps
• Sequencing results
  • K777000 in pSB1C3
  • K777001 in pSB1C3
  • K777002 in pSB1C3
  • K777003 in pSB1C3
  • K777004 in pSB1C3
  • K777005 in pSB1C3
  • K777006 in pSB1C3
  • K777007 in pSB1C3
  • K777008 in pSB1C3

flhDC

• K777100 in pSB1C3 ApE plasmid map

fliC

• K777109 in pSB1C3 ApE plasmid map

motA

• K777113 in pSB1C3 ApE plasmid map

motB

• K777117 in pSB1C3 ApE plasmid map

yhjH

• K777121 in pSB1C3 ApE plasmid map

Constitutive promoter + RFP-constructs

• K777125-K777132 ApE plasmid maps

Sequences

Primers

• Mutagenesis
• PCR
• QuikChange
• Sequencing
• Real-time

Raw Datasets

Characterization of constitutive promoters by qrtPCR

CP (crossing point) values and relative reinforcments of promoters from the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson Family]:J23100, J23104, J23105, J23106, J23109, J23112, J23113, J23114
The CP values ​​of the reference housekeeping genes (hkg) are reduced by the value of 3.32 for the dilution which have been used for the reference cDNA in the experiment.

Promoter		CP1	CP2	CP3	Average	Standard variance
J23113 (Calibrator)	J23113	23.7	24.46	24.51
	J23113_hkg	5.48	5.33	4.57
	ΔCT Calibrator (CP - CP_hkg)	18.22	18.93	19.94	19.03	0.71
J23106	J23106	23.25	22.37	22.44
	J23106_hkg	4.66	5.58	5.59
	ΔCT J23106	17.63	16.73	16.85
	ΔΔCT J23106	1.4	2.3	2.18
	Reinforcment	2.64	4.92	4.53	4.03	1.00
J23112	J23112	20.43	20.42	20.24
	J23112_hkg	5.00	4.9	5.2
	ΔCT J23112	15.43	15.52	15.04
	ΔΔCT J23112	3.6	3.51	3.99
	Reinforcment	12.13	11.39	15.89	13.14	1.97
J23114	J23112	20.35	20.07	20.88
	J23114_hkg	5.77	6.13	5.75
	ΔCT J23114	14.58	13.94	15.13
	ΔΔCT J23114	4.45	5.09	3.90
	Reinforcment	21.86	34.06	14.93	23.62	7.91
J23109	J23109	18.56	18.25	18.50
	J23109_hkg	5.79	5.43	5.91
	ΔCT J23109	12.77	12.82	12.59
	ΔΔCT J23109	6.26	6.21	6.44
	Reinforcment	76.64	74.03	86.82	79.16	5.52
J23105	J23105	16.05	16.21	16.13
	J23105_hkg	4.98	5.22	5.18
	ΔCT J23105	11.07	10.99	10.95
	ΔΔCT J23105	7.96	8.04	8.08
	Reinforcment	249.00	263.20	270.60	260.93	8.96
J23104	J23104	15.76	16.50	15.85
	J23104_hkg	4.66	5.58	5.03
	ΔCT J23104	11.10	10.92	10.82
	ΔΔCT J23104	7.93	8.11	8.21
	Reinforcment	243.88	276.28	296.11	272.09	21.53
J23100	J23100	14.02	13.53	14.21
	J23100_hkg	4.06	4.23	5.12
	ΔCT J23100	9.96	9.30	9.09
	ΔΔCT J23100	9.07	9.73	9.94
	Reinforcment	537.45	849.22	982.29	789.65	186.42

Here you can find the according excel sheet and a more detailed description of the results.

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