Team:Goettingen/week7-2

From 2012.igem.org

Revision as of 09:47, 22 September 2012 by Rosin (Talk | contribs)

Deutsch  / English 
<html7

<tbody>

Contents

<a name="week7#2">#2 Speed Improvement - 7th week</a>

<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a>

V06_11


V06_11_1 Miniprep of the new flhDC-promoter constructs

  • Experiment:
    Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.


V06_11_2 Chemical retransformation of the new flhDC-promoter constructs into E. coli (DH10B)

  • Experiment:
    For the chemical retransformation the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> was followed. The following constructs were transformed into E. coli:

    20G - #1
    20G - #2
    2G - #1
    2G - #2
    2G - #3
    20I - #2
    20I - #3
    18O - #2
    18O - #3
    18K - #1
    18K - #2
    18K - #3


  • Observations & Results:
    The retransformation was successful since all plates showed numeours colonies except the negative control.


V06_12


Preparation of over night cultures</i>

  • Experiment:
    In order to gain further plasmid material over night cultures of one colonie of all eight flhDC-promoter constructs were prepared for subsequent plasmid isolation.

    - 20E - #2
    - 20G - #1
    - 2G - #1
    - 20I - #2
    - 18M - #2
    - 18O - #2
    - 18K - #1
    - 18C - #2


    </td></tr> </table>

    V06_13


    V06_13_1 Miniprep of the new flhDC-promoter constructs

    • Experiment:
      Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.


    V06_13_2 Chemical transformation of pBAD-sfGFP into E. coli (DH10B)

    • Experiment:
      For the chemical transformation the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> was followed.
    • Observations & Results:
      The transformation was successful since all plates showed numeours colonies except the negative control.


    V06_14


    Preparation of over night cultures</i></i>

    • Experiment:
      Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.

      </td></tr> </table>

      V06_15


      Miniprep of pBAD-sfGFP</i></i>

Retrieved from "http://2012.igem.org/Team:Goettingen/week7-2"