Team:Goettingen/week7-2

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#2 Speed Improvement - 7th week

V06_11_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_11_2 Chemical retransformation of the new flhDC-promoter constructs into E. coli (DH10B)

Observations & Results:
The retransformation was successful since all plates showed numeours colonies except the negative control.

Preparation of over night cultures

V06_13_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_13_2 Chemical transformation of pBAD-sfGFP into E. coli (DH10B)

Experiment:
For the chemical transformation the standard protocol was followed.
Observations & Results:
The transformation was successful since all plates showed numeours colonies except the negative control.

Preparation of over night cultures

Experiment:
Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.

Miniprep of pBAD-sfGFP

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

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