Team:Goettingen/week8-2

V06_18

• Experiment:
  In order to gain further plasmid material of the new vector pSB1C3 was transformed into E. coli(DH10B) as described in the protocol.
  
• Observations & Results:
  The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.

V06_19

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
• Observations & Results:
  The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed.

• Experiment:
  The chemical transformation was conducted as described in the protocol, however, a lower chloramphenicol concentration was used than before.
  
• Observations & Results:
  The transformation was successful since all plates showed numeours colonoes except the negative control.

V06_14

• Experiment:
  Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.
  
  

V06_15

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.