Team:Goettingen/week2-3

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Team Goettingen

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#3 Chemoreceptor Library - 2nd Week

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V05_08

Amplification of tar gene

Experiment:
PCR was performed over night with the Tar forward and reverse primers using Pfu polymerase and the isolated genomic DNA from DH10B as template according to protocol.

V05_10

Amplification of tar gene

Experiment:
PCR was controlled by running a 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
Observations & Results:
A band at about 1.6 kb was detected. Amplification of tar was successful.

V05_11

Amplification of tar gene

Experiment:
PCR clean-up using peqGOLD Gel Extraction Kit according to the manual.

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