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Team:Bielefeld-Germany/Labjournal/week4
From 2012.igem.org
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** model the interesing parts of a clarification plant (the part witch are interesing for our cleaner. | ** model the interesing parts of a clarification plant (the part witch are interesing for our cleaner. | ||
| - | * '''Team Bacterial Laccases''': We did some restrictions to clone our laccase PCR products from ''Xanthomonas campestris'' and ''E. coli'' in pSB1C3 backbone. Therefore we transformed pSB1C3 with RFP in our competent KRX cells, picked colnies and made plasmidisolations. | + | * '''Team Bacterial Laccases''': We did some restrictions to clone our laccase PCR products from ''Xanthomonas campestris'' and ''E. coli'' in pSB1C3 backbone. Therefore we transformed pSB1C3 with RFP in our competent KRX cells, picked colnies and made plasmidisolations. After that we ligated the cutted products with the cutted plasmid backbone. |
===Tuesday May 22nd=== | ===Tuesday May 22nd=== | ||
Revision as of 08:34, 5 September 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18 Week19
Week 4 (05/21 - 05/27/12)
Monday May 21st
- Team Modeling: Our aims for modeling:
- model the expression of the laccases in the organisms.
- model the aktivity of our enzyms.
- model the interesing parts of a clarification plant (the part witch are interesing for our cleaner.
- Team Bacterial Laccases: We did some restrictions to clone our laccase PCR products from Xanthomonas campestris and E. coli in pSB1C3 backbone. Therefore we transformed pSB1C3 with RFP in our competent KRX cells, picked colnies and made plasmidisolations. After that we ligated the cutted products with the cutted plasmid backbone.
Tuesday May 22nd
- Successful PCRs of laccase genes from Thermus thermophilus HB27 and Bacillus halodurans C-125 with genomic DNA as template. The primers were designed with T7-Promotor-overhanging ends after prefix and HIS-Tag-overhang before suffix.
Wednesday May 23rd
- Team Student Academy: Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells. For setup see here. Got intense red fluorescing colonies but no green fluorescing colonies. Made a backup of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]. Asking for other plasmids containing GFP at the working groups of our University.
Thursday May 24th
- Team Student Academy: Made a liquid culture of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] at 30°C. There was no fluorescence. Furthermore [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] was transformed. Also got intense red fluorescing colonies.
