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Team:TMU-Tokyo
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| + | Our team project | ||
| + | <H1>Project Abstract</H1> | ||
Our project's goal is creating E.coli to resolve formaldehyde. | Our project's goal is creating E.coli to resolve formaldehyde. | ||
Formaldehyde is a toxic substance that exists close around our lives (cf. agricultural chemicals, antiseptics in hospital, paint of building materials, etc.) and effects negatively on human body even at a relatively low concentration. Moreover, mass production of formaldehyde is done at many factories these days. So it could easily happen that formaldehyde flows out environment over its permissible amount. In fact, an over limit concentration of formaldehyde was detected from Tone River and became a hot topic in Japan (May, 2012).In these points, we make a goal to create E.coli with ability to detoxification. E.coli has a small amount of formaldehyde dehydrogenase. And this enzyme change formaldehyde to formic acid. E.coli has an enzyme too to change formic acid to hydrogen and carbon dioxide. Thesefore, we plan to overexpress these two enzymes in E.coli. The degrading process by the 2 enzymes follows the formula below. | Formaldehyde is a toxic substance that exists close around our lives (cf. agricultural chemicals, antiseptics in hospital, paint of building materials, etc.) and effects negatively on human body even at a relatively low concentration. Moreover, mass production of formaldehyde is done at many factories these days. So it could easily happen that formaldehyde flows out environment over its permissible amount. In fact, an over limit concentration of formaldehyde was detected from Tone River and became a hot topic in Japan (May, 2012).In these points, we make a goal to create E.coli with ability to detoxification. E.coli has a small amount of formaldehyde dehydrogenase. And this enzyme change formaldehyde to formic acid. E.coli has an enzyme too to change formic acid to hydrogen and carbon dioxide. Thesefore, we plan to overexpress these two enzymes in E.coli. The degrading process by the 2 enzymes follows the formula below. | ||
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<font size="3" >There are several reasons why we use E.coli for detoxifying formaldehyde. First, cultivating E. coli can easily do mass production of the degrading enzymes. Second, this system can be industrially applied. For example, if an Apotosis function is attached to an E. coli that detoxifies formaldehyde, it can be used to purify particular contaminated environments and also tap water. Moreover, hydrogen is obtained as a degradation product of formic acid. Since hydrogen is expected as a new clean energy for fuel cell, it will also be a great industrial development if its recovery becomes possible from our formaldehyde degrading system by E. coli.</font> | <font size="3" >There are several reasons why we use E.coli for detoxifying formaldehyde. First, cultivating E. coli can easily do mass production of the degrading enzymes. Second, this system can be industrially applied. For example, if an Apotosis function is attached to an E. coli that detoxifies formaldehyde, it can be used to purify particular contaminated environments and also tap water. Moreover, hydrogen is obtained as a degradation product of formic acid. Since hydrogen is expected as a new clean energy for fuel cell, it will also be a great industrial development if its recovery becomes possible from our formaldehyde degrading system by E. coli.</font> | ||
Revision as of 05:08, 3 September 2012
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Our team project
Project Abstract
Our project's goal is creating E.coli to resolve formaldehyde. Formaldehyde is a toxic substance that exists close around our lives (cf. agricultural chemicals, antiseptics in hospital, paint of building materials, etc.) and effects negatively on human body even at a relatively low concentration. Moreover, mass production of formaldehyde is done at many factories these days. So it could easily happen that formaldehyde flows out environment over its permissible amount. In fact, an over limit concentration of formaldehyde was detected from Tone River and became a hot topic in Japan (May, 2012).In these points, we make a goal to create E.coli with ability to detoxification. E.coli has a small amount of formaldehyde dehydrogenase. And this enzyme change formaldehyde to formic acid. E.coli has an enzyme too to change formic acid to hydrogen and carbon dioxide. Thesefore, we plan to overexpress these two enzymes in E.coli. The degrading process by the 2 enzymes follows the formula below. Formaldehyde HCHO -> Formic acid HCOOH -> CO2 + H2O
There are several reasons why we use E.coli for detoxifying formaldehyde. First, cultivating E. coli can easily do mass production of the degrading enzymes. Second, this system can be industrially applied. For example, if an Apotosis function is attached to an E. coli that detoxifies formaldehyde, it can be used to purify particular contaminated environments and also tap water. Moreover, hydrogen is obtained as a degradation product of formic acid. Since hydrogen is expected as a new clean energy for fuel cell, it will also be a great industrial development if its recovery becomes possible from our formaldehyde degrading system by E. coli.
File:TMU-Tokyo logo.png 200px | |
File:TMU-Tokyo team.png Your team picture | |
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