Team:Paris Bettencourt/Suicide
From 2012.igem.org
(Difference between revisions)
(→Overview) |
(→Objectives) |
||
Line 8: | Line 8: | ||
==Objectives== | ==Objectives== | ||
- | + | #Design an anti-toxin part that confers immunity against the colicin E2 toxin | |
- | + | #Develop RFP labeled MG1655 Z1 strains immune to colicin E2 toxin | |
+ | #Measure viability of immune and vulnerable MG1655 Z1 cells in the presence of WT ColE2 cells | ||
+ | #Design a colicin E2 toxin part under the control of a constitutive promoter and establish in immune MG1655 Z1 cells to generate synthetic ColE2 cells | ||
+ | #Measure viability of immune and vulnerable MG1655 Z1 cells in the presence of synthetic ColE2 cells | ||
+ | #Tune the concentrations of toxin and anti-toxin | ||
+ | #Combine our system with the Restriction Enzyme Group`s work, measure viability | ||
==Design== | ==Design== |
Revision as of 17:57, 28 August 2012
Title
Contents |
Overview
Our goal is to engineer a synthetic toxin-antitoxin system from the wild type colicin E2. This synthetic toxin-antitoxin system is species specific, will allow for population level cell death, destruction of the genetic material, and will function on a tunable delay.
Objectives
- Design an anti-toxin part that confers immunity against the colicin E2 toxin
- Develop RFP labeled MG1655 Z1 strains immune to colicin E2 toxin
- Measure viability of immune and vulnerable MG1655 Z1 cells in the presence of WT ColE2 cells
- Design a colicin E2 toxin part under the control of a constitutive promoter and establish in immune MG1655 Z1 cells to generate synthetic ColE2 cells
- Measure viability of immune and vulnerable MG1655 Z1 cells in the presence of synthetic ColE2 cells
- Tune the concentrations of toxin and anti-toxin
- Combine our system with the Restriction Enzyme Group`s work, measure viability
Design
Present the design of your system, both in a written form, and a schematic one.
Experiments and results
Characterisation of X
Experimental setup
Describe the experiment
Results
Present your results
Testing of the system
Experimental setup
Describe the experiment
Results
Present your results