Revision as of 12:05, 22 August 2012

Labjournal

Primerdesign for isolating a laccase from Arababidopsis thaliana cDNA. Since we want to express the laccase in E.coli we designed the primers like before for the bacterial laccases with T7 promotor and HIS-tag.
Team bacterial laccase: The bacteria S. griseus and S. lavendulae has been delivered so we can start with PCRs.

Team Activity Tests: After testing the T. versicolor laccase under conditions that are optimal (pH 5, 25°C ) according to the literature we now started further characterization under different pHs. We analyzed the laccases behavior when working in 100 mM natrium acetate buffer at pH 1, 3, 7 an 9. Result: We agree with the literature that pH 5 and also pH 6 seem to make the laccase happy. Since not all waste waters (especially those here in Germany) are not as warm as 25°C we now wonder what our laccase might do when exposed to low temperature such as 4°C. Stay tuned.

Team Wiki: Yay for Team Wiki´s first entry. Our first steps with the iGEM Bielefeld 2012 Wiki contain thinking about contents, layouts, programming and responsibilities. Our first rules are:
- we are programming static pages in HTML and all the other pages (those that will be updated by all team members) in wiki code.
- we created all pages and will fill them up with some nice and beautiful content constantly from now on.
- Our temporary banner contains our outstanding logo and a DNA but we will set up a new layout soon.

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 * Primerdesign for isolating a laccase from ''Arababidopsis thaliana'' cDNA. Since we want to express the laccase in E.coli we designed the primers like before for the bacterial laccases with T7 promotor and HIS-tag.
+* '''Team bacterial laccase''': The bacteria ''S. griseus'' and ''S. lavendulae'' has been delivered so we can start with PCRs.
 ===Tuesday June 5th===