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Team:University College London/Protocols/ColonyDNAExtraction
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Latest revision as of 09:42, 22 August 2012
DNA Extraction from Colonies
Step 1 - Picking Colony: Using a pipette select an isolated colony
Step 2 - Deposit: Dip the colony in 10ul of dH20, preferably in screw top container
Step 3 - Boiling Stage:Boil the dH20 100 for 5-10mins
Step 4 - Centrifuge:
Time: 1 min
RPM: 8000
Temp: 18oC
Step 5 - Remove Supernatant: The supernatant contains the DNA template.
