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Team:University College London/Protocols/ColonyDNAExtraction
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<noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} | <noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} | ||
| - | == | + | == DNA Extraction from Colonies == |
</noinclude> | </noinclude> | ||
'''Step 1 - Picking Colony:''' Using a pipette select an isolated colony | '''Step 1 - Picking Colony:''' Using a pipette select an isolated colony | ||
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'''Step 2 - Deposit:''' Dip the colony in 10ul of dH20, preferably in screw top container | '''Step 2 - Deposit:''' Dip the colony in 10ul of dH20, preferably in screw top container | ||
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'''Step 3 - Boiling Stage:'''Boil the dH20 100 for 5-10mins | '''Step 3 - Boiling Stage:'''Boil the dH20 100 for 5-10mins | ||
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'''Step 4 - Centrifuge:''' | '''Step 4 - Centrifuge:''' | ||
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Time: 1 min | Time: 1 min | ||
RPM: 8000 | RPM: 8000 | ||
Temp: 18oC | Temp: 18oC | ||
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'''Step 5 - Remove Supernatant:''' The supernatant contains the DNA template. | '''Step 5 - Remove Supernatant:''' The supernatant contains the DNA template. | ||
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Revision as of 09:41, 22 August 2012
DNA Extraction from Colonies
Step 1 - Picking Colony: Using a pipette select an isolated colony
Step 2 - Deposit: Dip the colony in 10ul of dH20, preferably in screw top container
Step 3 - Boiling Stage:Boil the dH20 100 for 5-10mins
Step 4 - Centrifuge:
Time: 1 min RPM: 8000 Temp: 18oC
Step 5 - Remove Supernatant: The supernatant contains the DNA template.
