Revision as of 19:29, 19 August 2012

Labjournal

Team Modeling: programming our first differential equation and finding the ODE15s function witch solves these equations.
Team Cellulose Binding Domain: Looked for the sequence of Clostridium cellulovorans cellulose binding protein gene (cbp A) http://www.ncbi.nlm.nih.gov/nuccore/M73817 made a Clonemanagerfile and protein-BLASTed the sequence to find out the domain of the Cellulose-Binding-Domain: 103bp-378bp

Team Activity Tests: Since our Tecan microplate reader is not able to actively cool down to 4 °C we got the chance to meet the photometer Carry. Check "protocols" for further information about her. We used the same set up with 100 mM natrium acetate buffer, 0,1 U T. versicolor laccase and 0,1 mM ABTS as before but now measured at 4°C. Our team is planning to visit a municipal sewage plant for getting some insights into the water conditions there, so we will for sure test other temperatures after having more information. Let´s hope the water there is a little warmer since laccase doesn´t seem to be totally satisfied at 4°C. I wouldn´t either.

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 ===Tuesday June 12th===
-* '''Team Site Directed Mutagenesis:''' Generated the first Site-Directed-Mutagenesis primer for Bacillus pumilus (two sites), Xanthomonas Campestris (two sites), Escherichia coli (one site)und Thermus thermophilus (one site).
-* '''Team Cellulose Binding Domain:''' Searched NCBI for Cellulose, Chitin & Keratin binding motifs in accessable organisms; found two Chitin-binding-domains in Bacillus Halodurans
 ===Wednesday June 13th===