BioBricks

Module 1: Plastic Detection

Figure 1: The figure above shows our genetic circuit for the Detection Module. The Constitutive Promoter (BBa_J23119) drives constant expression of the NahR protein BBa_K228004 (modified - see below). NahR is a transcriptional activator, which is activated in response to salicylate molecules. Our proxy for plastic - Persistant Organic Pollutants - have a salicylate-like domain, and are therefore capable of activating NahR. Activated NahR binds the p(sal) promoter (Figure 2) thus activating the circuit for Module 2 (Aggregation).

Modified BBa_K228004: The BBa_K228004 BioBrick must be modified as it combines both the NahR protein and the p(sal) promoter. We wish to seperate these.

Please see our Detection Description Subpage for more details on this Module.

Module 2: Aggregation

Figure 2: The Figure above illustrates our circuit for Module 2 (Aggregation). The p(sal) Promoter BBa_K228004 (modified - see below) which triggers the Aggregation circuit is turned on when bound by activated NahR protein from Module 1. Once the p(sal) promoter is activated, it drives expression of the Curli Cluster of genes (BBa_K729003). The individuals genes of the cluster are shown radiating from BBa_K540000.

Modified BBa_K228004: The BBa_K228004 BioBrick must be modified as it combines both the NahR protein and the p(sal) promoter. We wish to seperate these.

BBa_K729003: This BioBrick is modified from BBa_K540000, which has a cobalt promoter.

For more information on how this circuit is triggered, and the individual roles of the Curli genes, please visit our Aggregation Description Subpage

Module 3: Plastic Degradation

Figure 3: The image above shows the circuit for Module 3 (Degradation). The Constitutive Promoter (BBa_J23119) drives constant expression of the Laccase BioBrick (BBa_K729002). The Laccase gene we intend to use originates from a particular bacterial strain, and is capable of Polyethylene Degradation.

For more information on how this circuit functions, please visit our Degradation Description Subpage

Module 4: Buoyancy

We have designed a composite BioBrick which comprises all of these parts called BBa_K729000

For more information on how this circuit functions, please visit our Buoyancy Description Subpage

Module 5: Salt Tolerance

Figure 5: The image above illustrates the circuit design for Module 5 (Salt Tolerance). The Constitutive Promoter (BBa_J23119) will drive constant expression of our novel BioBrick irre, which confers salt resistance onto E.coli.

For more information on how this circuit functions, please visit our Salt Tolerance Description Subpage

Module: Containment

Figure 6: The above image shows the genetic circuit for our threefold Containment module. This involves two separate plasmids. The first plasmid carries the toxins Holin/Endolysin (BBa_K729009) and Colicin E3 (BBa_K729005), as well as the secreted nuclease EcoRI (BBa_K729006). The second plasmid carries the antitoxins Anti-Holin (BBa_K729010) and Colicin E3 Immuntiy (BBa_K729008).

For more information on how this circuit functions, please visit our Containment Description Subpage