BioBricks

Module: Plastic Detection

Figure 1: The figure above shows our genetic circuit for the Detection Module. The Constitutive Promoter (BBa_J23119) drives constant express of the NahR protein. NahR (BBa_K228004) is a transcriptional activator, which is activated in response to salicylate molecules. Our proxy for plastic - Persistant Organic Pollutants - have a salicylate-like domain, and are therefore capable of activating NahR. Activated NahR binds the p(sal) promoter (Figure 2) thus activating the circuit for Module 2 (Aggregation).

Please see our Detection Description page for more details.

Module: Aggregation

Module: Plastic Degradation

BBa_K729002 Laccase for Polyethylene Degradation Laccase for the degradation of polyethylene and organic pollutants.

Module: Buoyancy

BBa_K729000 Temperature regulated Gas Vesicle Polycistronic gene cluster composite This composite consists of the gas vesicle polycistronic gene regulated by heat shock promoter BBa_K338001, repressor tetR and corresponding promoter P(TetR), such that a reduction in temperature induces greater expression of the gvp gene cluster. Activation of Heat Shock Promoter, triggers expression of the repressor protein tetR, which binds and repressors the promoter p(TetR). p(TetR) is the promoter driving constitutive gas vesicle formation, and so activation of the repressor system down regulates gas vesicle formation.

Module: Salt Tolerance

BBa_K729001 irrE form Deinococcus radiodurans confers salinity and osmotic stress tolerance irrE confers salt tolerance in E. coli. Sequence pending

Module: Containment

BBa_K729010, BBa_K729009 Three fold active biological containment System