Team:HokkaidoU Japan/Notebook/plastic Week 2

From 2012.igem.org

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Today we made media of pGEM transformed JM109 to produce PHB.
 
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To make JM109 produce PHB、we have pre-culture with a normal media before starting culture with
 
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polymer-making media.
 
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Transformation of pGEM into JM109. This transformation is the '''bioplastic production test in JM109'''.
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#Added 1 ul of plasmid DNA to 50 ul of thawed competent cells on ice.
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#Incubated on ice for 30 min.
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#Added 600 ul of LB.
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#Prepared and Labeled two petri dishes with LBA.
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#Plate 300 ul of the transformation onto LBA dish and spread.
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#Added 900 ul of LB to 100 ul of the transformation and plated 300 ul of it onto LBA dish then spread.
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#Incubated the plates at 37C for 15hrs 30 min.
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Revision as of 09:12, 11 August 2012

July 9th

Transformation of pGEM into JM109. This transformation is the bioplastic production test in JM109.

  1. Added 1 ul of plasmid DNA to 50 ul of thawed competent cells on ice.
  2. Incubated on ice for 30 min.
  3. Added 600 ul of LB.
  4. Prepared and Labeled two petri dishes with LBA.
  5. Plate 300 ul of the transformation onto LBA dish and spread.
  6. Added 900 ul of LB to 100 ul of the transformation and plated 300 ul of it onto LBA dish then spread.
  7. Incubated the plates at 37C for 15hrs 30 min.


July 10th

How to make pGEM

Cultivaton




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