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Team:HokkaidoU Japan/Notebook/plastic Week 5
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| + | <div class="hokkaidou-notebook-daily"> | ||
| + | ==August 2nd== | ||
| + | <div> | ||
| + | <p> | ||
| + | ==Transformation== | ||
| + | <p> | ||
| + | Transformation of pGEM into BL21. This transformation is the '''plastic production test in BL21'''. | ||
| + | |||
| + | #Added 1ul of plasmid DNA to 50ul of thawed competent cells on ice. | ||
| + | #Incubated on ice for 30min. | ||
| + | #Added 600ul of LB. | ||
| + | #Prepared and Labeled two petri dishes with LBA. | ||
| + | #Plate 300ul of the transformation onto LBA dish and spread. | ||
| + | #Added 900ul of LB to 100ul of the transformation and plated 300ul of it onto LBA dish then spread. | ||
| + | #Incubated the plates at 37C for OOhrs. | ||
| + | </p> | ||
| + | |||
| + | </div><div> | ||
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Revision as of 03:55, 3 August 2012
August 2nd
Transformation
<p> Transformation of pGEM into BL21. This transformation is the plastic production test in BL21.
- Added 1ul of plasmid DNA to 50ul of thawed competent cells on ice.
- Incubated on ice for 30min.
- Added 600ul of LB.
- Prepared and Labeled two petri dishes with LBA.
- Plate 300ul of the transformation onto LBA dish and spread.
- Added 900ul of LB to 100ul of the transformation and plated 300ul of it onto LBA dish then spread.
- Incubated the plates at 37C for OOhrs.
