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Team:University College London/Protocols/Competency2
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'''Step 1 -Creating culture media:''' In a sterile environment, set up a falcons, with 5mls of Lysogeny Broth and 100ul 1M MgS04. | '''Step 1 -Creating culture media:''' In a sterile environment, set up a falcons, with 5mls of Lysogeny Broth and 100ul 1M MgS04. | ||
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'''Step 2 – Selecting a Colony:''' Select a clear, isolated colony and using an inoculation hoop, scoop up a colony onto the tip. Deposit in the falcon tube | '''Step 2 – Selecting a Colony:''' Select a clear, isolated colony and using an inoculation hoop, scoop up a colony onto the tip. Deposit in the falcon tube | ||
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'''Step 3 - Cell culture:''' Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours. | '''Step 3 - Cell culture:''' Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours. | ||
Latest revision as of 09:59, 2 August 2012
Generating Competency Day 2
Step 1 -Creating culture media: In a sterile environment, set up a falcons, with 5mls of Lysogeny Broth and 100ul 1M MgS04.
Step 2 – Selecting a Colony: Select a clear, isolated colony and using an inoculation hoop, scoop up a colony onto the tip. Deposit in the falcon tube
Step 3 - Cell culture: Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours.
