Team:University College London/Protocols/Nanodrop

From 2012.igem.org

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Model:
Model:
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Step 1: Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software
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'''Step 1:''' Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software
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Step 2: Wipe and add elution buffer as negative control. Click blank in ND-1000 software
+
'''Step 2:''' Wipe and add elution buffer as negative control. Click blank in ND-1000 software
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Step 3: Wipe and add 1 µl sample
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'''Step 3:''' Wipe and add 1 µl sample
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Step 4: On the software set lambda to 260nm
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'''Step 4:''' On the software set lambda to 260nm
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Step 5: Lower the lever arm and click measure in ND-1000 software
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'''Step 5:''' Lower the lever arm and click measure in ND-1000 software
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Step 6: Take readings for concentration and purity
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'''Step 6:''' Take readings for concentration and purity
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Step 7: Once measurement complete, wipe surface
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'''Step 7:''' Once measurement complete, wipe surface
{{:Team:University_College_London/templates/foot}}
{{:Team:University_College_London/templates/foot}}

Revision as of 09:53, 1 August 2012

Nanodrop

Software ND-1000 Model:

Step 1: Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software

Step 2: Wipe and add elution buffer as negative control. Click blank in ND-1000 software

Step 3: Wipe and add 1 µl sample

Step 4: On the software set lambda to 260nm

Step 5: Lower the lever arm and click measure in ND-1000 software

Step 6: Take readings for concentration and purity

Step 7: Once measurement complete, wipe surface

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