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Team:University College London/Protocols/Nanodrop
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| - | 1 | + | Step 1: Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software |
| - | 2 | + | Step 2: Wipe and add elution buffer as negative control. Click blank in ND-1000 software |
| - | 3 | + | Step 3: Wipe and add 1 µl sample |
| - | 4 | + | Step 4: On the software set lambda to 260nm |
| - | 5 | + | Step 5: Lower the lever arm and click measure in ND-1000 software |
| - | 6 | + | Step 6: Take readings for concentration and purity |
| - | 7 | + | Step 7: Once measurement complete, wipe surface |
{{:Team:University_College_London/templates/foot}} | {{:Team:University_College_London/templates/foot}} | ||
Revision as of 09:52, 1 August 2012
Nanodrop
Software ND-1000 Model:
Step 1: Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software
Step 2: Wipe and add elution buffer as negative control. Click blank in ND-1000 software
Step 3: Wipe and add 1 µl sample
Step 4: On the software set lambda to 260nm
Step 5: Lower the lever arm and click measure in ND-1000 software
Step 6: Take readings for concentration and purity
Step 7: Once measurement complete, wipe surface
