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Team:University College London/Protocols/RestrictionEnzymeDigest1
From 2012.igem.org
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== Enzyme Digest == | == Enzyme Digest == | ||
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1. Thaw all materials on ice | 1. Thaw all materials on ice | ||
Revision as of 16:06, 31 July 2012
Enzyme Digest
1. Thaw all materials on ice
2. Add the following ingredients to autoclaved/sterile eppendorf tubes
Insert Table
3.Flick contents gently and centrifuge.
4.To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight
5. Then run on the following conditions (by turning the dial)
RPM: 14000
Time: 1 minute
Temperature: 18oC
