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Team:University College London/Protocols/Nanodrop
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Software ND-1000 | Software ND-1000 | ||
Revision as of 15:32, 16 July 2012
== Nanodrop ==
Software ND-1000 Model:
1.Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software
2.Wipe and add elution buffer as negative control. Click blank in ND-1000 software
3.Wipe and add 1 µl sample
4.On the software set lambda to 260nm
5.Lower the lever arm and click measure in ND-1000 software
6.Take readings for concentration and purity
7.Once measurement complete, wipe surface
