Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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Revision as of 20:29, 26 October 2012

Project Achievements

We successfully produced purified and identified active laccases of E. coli BL21(CueO named ECOL), Bacillus pumilus DSM 27 laccase (CotA named BPUL), Bacillus halodurans C-125 laccase (Lbh1 named BHAL) and Thermus thermophilus HB27 (tthL named TTHL).
Results ECOL
Results BPUL
Results TTHL
Results BHAL

We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. We were able to show that the vector is functioning as expected.
Results

We successfully produced the laccase TVEL5 from Trametes versicolor in P. pastoris and showed the activity of the produced enzymes.
Results

Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.
Results ECOL
Results BPUL
Results TTHL
Results BHAL

We established a method for the degradation of several compounds with the help of our self-produced laccases. The HPLC analysis showed that our produced bacterial laccases are able to degrade estradiol with and without addition of ABTS. The laccase BPUL is additionally able to degrade ethinyl estradiol with addition of ABTS.
Results

We successfully immobilized Trametes versicolor laccase (named TVEL0), E. coli BL21 laccase (CueO named ECOL) and Bacillus pumilus DSM 27 laccase (CotA named BPUL) and measured their activity.
Results TVEL0
Results ECOL
Results BPUL

We successfully improved a BioBrick by clarifying its true function.
[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]

We successfully created our own database to organize our samples.
Results

We successfully created a model which simulates the oxidation of ABTS.
Results

We conducted a feasibility study of our project with sewage plant experts.
Results

We did an outstanding outreach work.
Results

We won a gold medal.

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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

Contact Us

Universität Bielefeld
Universitätsstraße 25
D-33615 Bielefeld

info(at)igem-bielefeld.de

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@@ Line 26: / Line 26: @@
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest.
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. We were able to show that the vector is functioning as expected.
 [https://2012.igem.org/Team:Bielefeld-Germany/Results/vector Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced the laccase TVEL5 from ''Trametes versicolor'' in ''P. pastoris'' and showed the activity of the produced enzymes.
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 Results]
 [[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.
@@ Line 41: / Line 43: @@
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We established a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analysis showed that our laccases are able to degrade the hormonal substrates estradiol, estrone and ethinyl estradiol.
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We established a method for the degradation of several compounds with the help of our self-produced laccases. The HPLC analysis showed that our produced bacterial laccases are able to degrade estradiol with and without addition of ABTS. The laccase BPUL is additionally able to degrade ethinyl estradiol with addition of ABTS.
 [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor''-laccase (named TVEL0), ''E. coli'' BL21-laccase (CueO named ECOL) and ''Bacillus pumilus'' DSM 27-laccase (CotA named BPUL) and measured their activity.
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor'' laccase (named TVEL0), ''E. coli'' BL21 laccase (CueO named ECOL) and ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL) and measured their activity.
 [https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results TVEL0]