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Team:Bielefeld-Germany/Amsterdam/Results
From 2012.igem.org
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Revision as of 19:22, 26 October 2012
Summary
coming soon
Laccases
The iGEM Team successfully produced again four active bacterial laccases:
All of the bacterial laccases were accomplished to purify. The purified laccases . Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol. The laccase of TVEL0 fromTrametes versicolor was produced and tested for activity on oaxidation of ABTS.
Subtrate Analysis
Since Amsterdam team activity test, team immobilization and team substrate analysis used laccases from the same pool. With these laccases we repeated the degradation experiments. For BPUL and ECOL more biological replicates were measured and the laccases BHAL and TTHL were characterized for estradiol and ethinyl estradiol degradation. Possible resulting degradation products were futher analysed via LCMS-MS.
For more informations click here
Cellulose binding domain
A lot of efforts were made, to change the order of the fusion proteins, to change the promoter and the RBS and to insert a different linker between the cellulose binding domain and the reporter GFP, but to the last day of lab work no green glowing colony to work with and execute the binding assay could be generated.
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