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Team:TU-Delft/Snifferometer
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= Design of Snifferometer = | = Design of Snifferometer = | ||
| - | == | + | == The Concept of the Device == |
| + | [[File:Scheme of snifferometer.png|270px|right|thumb|'''Figure 1''': Scheme of Snifferometer]] | ||
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Figure 1 shows the concept of how the device works. The LEDs emit blue light on the liquid mixing Snifferomyces and sputum of the patient. Later, the photodiode collects the glowing light and transfers it into an amplified signal of voltage. Thus, by reading the number of voltmeter, the status of TB smelling cells in sputum can be detected. | Figure 1 shows the concept of how the device works. The LEDs emit blue light on the liquid mixing Snifferomyces and sputum of the patient. Later, the photodiode collects the glowing light and transfers it into an amplified signal of voltage. Thus, by reading the number of voltmeter, the status of TB smelling cells in sputum can be detected. | ||
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<div id="contentbox" style="text-align:justify;"> | <div id="contentbox" style="text-align:justify;"> | ||
| - | == | + | == Implementation of Snifferometer == |
| + | [[File:snifferometer device.png|250px|right|thumb|'''Figure 2''': Real Snifferometer]] | ||
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[[File:Circuit of Snifferometer.png|580px|Right|thumb|'''Figure 3''': Circuit of Snifferometer]] | [[File:Circuit of Snifferometer.png|580px|Right|thumb|'''Figure 3''': Circuit of Snifferometer]] | ||
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Revision as of 14:39, 26 October 2012
In order to assess the feasibility using our Snifferomyces cells as a diagnosis method, a device called "Snifferometer" was built to measure the status of fluorescence. The purpose of building such a device is to overcome the complexity, incalculability, and the high cost of using fluorescence microscope.
Contents |
Design of Snifferometer
The Concept of the Device
Figure 1 shows the concept of how the device works. The LEDs emit blue light on the liquid mixing Snifferomyces and sputum of the patient. Later, the photodiode collects the glowing light and transfers it into an amplified signal of voltage. Thus, by reading the number of voltmeter, the status of TB smelling cells in sputum can be detected.
Implementation of Snifferometer
Test
Eppendorf tubes are used to contain the liquid.
Two filters were tested:
- [http://www.leefilters.com/lighting/colour-details.html#768 LEE Filters 768 Egg yellow].
- [http://www.leefilters.com/lighting/colour-details.html#101 LEE Filters 101 yellow].
Four sets
- Control: M9 medium with E-coli
- Test: M9 medium with E-coli overexpressing TagGFP2
M9 medium is used because it has low autofluorescence.
- reduce background light intensity: right filter
- increase green light intensity
- increase the sensitivity of the circuit
- enlarge the area of fluorescent
