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Team:Bielefeld-Germany/Project/Achievments
From 2012.igem.org
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully produced purified and identified active laccases of ''E. coli'' BL21(CueO named ECOL), ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL), ''Bacillus halodurans'' C-125 laccase (Lbh1 named BHAL) and ''Thermus thermophilus'' HB27 (tthL named TTHL). | [[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully produced purified and identified active laccases of ''E. coli'' BL21(CueO named ECOL), ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL), ''Bacillus halodurans'' C-125 laccase (Lbh1 named BHAL) and ''Thermus thermophilus'' HB27 (tthL named TTHL). | ||
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/coli Results ECOL] |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results BPUL] |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo Results TTHL] |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/halo Results BHAL] |
[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. | [[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. | ||
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions. | [[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions. | ||
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/coli#Activity_Analysis_of_ECOL Results ECOL] |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi#Activity_analysis_of_BPUL Results BPUL] |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo#Activity_analysis_of_TTHL Results TTHL] |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/halo#Activity_Analysis_of_BHAL Results BHAL] |
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor''-laccase (named TVEL0), ''E. coli'' BL21-laccase (CueO named ECOL) and ''Bacillus pumilus'' DSM 27-laccase (CotA named BPUL) and measured their activity. | [[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor''-laccase (named TVEL0), ''E. coli'' BL21-laccase (CueO named ECOL) and ''Bacillus pumilus'' DSM 27-laccase (CotA named BPUL) and measured their activity. | ||
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results TVEL0] |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results ECOL] |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Results | + | [https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results BPUL] |
Revision as of 18:02, 23 October 2012
Project Achievements
Results ECOL Results BPUL Results TTHL Results BHAL
Results ECOL Results BPUL Results TTHL Results BHAL
Results TVEL0 Results ECOL Results BPUL
[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
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