Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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Revision as of 17:59, 23 October 2012

Project Achievements

We successfully produced purified and identified active laccases of E. coli BL21(CueO named ECOL), Bacillus pumilus DSM 27 laccase (CotA named BPUL), Bacillus halodurans C-125 laccase (Lbh1 named BHAL) and Thermus thermophilus HB27 (tthL named TTHL).
ECOL/coli Results BPUL/pumi Results TTHL/thermo Results BHAL/halo Results

We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest.
Results

Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.
ECOL/coli#Activity_Analysis_of_ECOL Results BPUL/pumi#Activity_analysis_of_BPUL Results TTHL/thermo#Activity_analysis_of_TTHL Results BHAL/halo#Activity_Analysis_of_BHAL Results

We established a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analysis showed that our laccases are able to degrade the hormonal substrates estradiol, estrone and ethinyl estradiol.
Results

We successfully immobilized Trametes versicolor-laccase (named TVEL0), E. coli BL21-laccase (CueO named ECOL) and Bacillus pumilus DSM 27-laccase (CotA named BPUL) and measured their activity.
TVEL0/immo Results ECOL/immo Results BPUL/immo Results

We successfully improved a BioBrick by clarifying its true function.
[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]

We successfully created our own database to organize our samples.
Results

We successfully created a model which simulates the oxidation of ABTS.
Results

We conducted a feasibility study of our project with sewage plant experts.
Results

We did an outstanding outreach work.
Results

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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

Contact Us

Universität Bielefeld
Universitätsstraße 25
D-33615 Bielefeld

info(at)igem-bielefeld.de

Navigate our Wiki

@@ Line 15: / Line 15: @@
 <b>
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced purified and identified an active ''E. coli'' BL21-Laccase (CueO named ECOL).
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced purified and identified active laccases of ''E. coli'' BL21(CueO named ECOL), ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL), ''Bacillus halodurans'' C-125 laccase (Lbh1 named BHAL) and ''Thermus thermophilus'' HB27 (tthL named TTHL).
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results ECOL/coli Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results BPUL/pumi Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results TTHL/thermo Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results BHAL/halo Results]
 [[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest.
@@ Line 23: / Line 25: @@
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced, purified and identified an active ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL).
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results ECOL/coli#Activity_Analysis_of_ECOL Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results BPUL/pumi#Activity_analysis_of_BPUL Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results TTHL/thermo#Activity_analysis_of_TTHL Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced, purified  an active laccase from ''Thermus thermophilus'' HB27 (tthL named TTHL).
+[https://2012.igem.org/Team:Bielefeld-Germany/Results BHAL/halo#Activity_Analysis_of_BHAL Results]
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced an active ''Bacillus halodurans'' C-125 laccase (Lbh1 named BHAL).
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/halo Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful characterization of the activity of ''B. pumilus'' laccase regarding the oxidization of ABTS under different conditions.
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi#Activity_analysis_of_BPUL Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful characterization of the activity of ''E. coli'' Laccase regarding the oxidization of ABTS under different conditions.
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli#Activity_Analysis_of_ECOL Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful measurements of enzyme activity of ''T. thermophilus'' laccase.
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo#Activity_analysis_of_TTHL Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful measurements of enzyme activity of ''B. halodurans'' laccase.
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/halo#Activity_Analysis_of_BHAL Results]
 [[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We established a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analysis showed that our laccases are able to degrade the hormonal substrates estradiol, estrone and ethinyl estradiol.
@@ Line 55: / Line 36: @@
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor''-laccase (named TVEL0) and measured their activity.
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor''-laccase (named TVEL0), ''E. coli'' BL21-laccase (CueO named ECOL) and ''Bacillus pumilus'' DSM 27-laccase (CotA named BPUL) and measured their activity.
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results TVEL0/immo Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results ECOL/immo Results]
+[https://2012.igem.org/Team:Bielefeld-Germany/Results BPUL/immo Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''E. coli'' BL21-laccase (CueO named ECOL) and measured their activity.
-[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Bacillus pumilus'' DSM 27-laccase (CotA named BPUL) and measured their activity. [https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results]