Team:Valencia/BG-11Wl

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Reagents and Materials

• Concentration BG-11 200X*:
• 8 g/l de K₂HPO₄•3H₂O.
• 8 g/l de Na₂CO₃.
• 15 g/l de MgSO₄•7H₂O.
• 0,2 g/l de Na₂EDTA.
• 7,2 g/l de CaCl₂•2H₂O.
• 1,2 g/l de ammonium Iron (III) citrate brown.
• 1,2 g/l de citric acid anhydrous.
• Concentration trace metal 1000X*
• 2,86 g/l de H₃BO₄.
• 1,81 g/l de MnCl₂•4H₂O.
• 222 mg/l de ZnSO₄•7H₂O.
• 390 mg/l de Na₂MoO₄•2H₂O.
• 79 mg/l de CuSO₄•5H₂0.
• 49 mg/l de CoCl₂•6H₂O.

Protocol

1. Add all the compounds for the BG-11 in an Erlenmeyer flask with 1 liter of distilled water and shake at room temperature until all salts are dissolved.
2. Add all the compounds for the trace metal in an Erlenmeyer flask with 1 liter of distilled water and shake at room temperature until all salts are dissolved.
3. In 1 liter of distilled water, put 1 Ml of trace metal and 5 ml of BG-11 and add 17’5 Mm of NaNO₃.
4. Autoclave at 121ºC during 20 minutes.
5. Add 10 Mm of Hepes of NaOH to set the Ph=7’8 (1 M Hepes NaOH = 700 Ml distilled water, 283’3 g Hepes, ≈ 5’5 g NaOH).