Team:Paris Bettencourt/Overview

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1) Physical containment: Our cells will be encapsulated with Arabinose and LB in order to avoid release of DNA/cells and to permit growth

1) Physical containment: Our cells will be encapsulated with Arabinose and LB in order to avoid release of DNA/cells and to permit growth

2) Semantic Containment.blablablablabla

1) Physical containment: Our cells will be encapsulated with Arabinose and LB in order to avoid release of DNA/cells and to permit growth

2) Semantic Containment.blablablablabla

3)Delay system: In the presence of Arabinose, LacI is produced repressing the expression of a restriction enzyme. Once Arabinose is not present anymore, the LacI repressor concentration decreases with dilution and degradation leading to the expression of the restriction enzyme.

1) Physical containment: Our cells will be encapsulated with Arabinose and LB in order to avoid release of DNA/cells and to permit growth

2) Semantic Containment.blablablablabla

3)Delay system: In the presence of Arabinose, LacI is produced repressing the expression of a restriction enzyme. Once Arabinose is not present anymore, the LacI repressor concentration decreases with dilution and degradation leading to the expression of the restriction enzyme.

4)Restriction Enzyme system: The restriction enzyme destroys a plasmid carrying an anti-toxin.

1) Physical containment: Our cells will be encapsulated with Arabinose and LB in order to avoid release of DNA/cells and to permit growth

2) Semantic Containment.blablablablabla

3)Delay system: In the presence of Arabinose, LacI is produced repressing the expression of a restriction enzyme. Once Arabinose is not present anymore, the LacI repressor concentration decreases with dilution and degradation leading to the expression of the restriction enzyme.

4)Restriction Enzyme system: The restriction enzyme destroys a plasmid carrying an anti-toxin.

5)Suicide system: Once the antitoxin is below a given threshold the toxin is no longer inhibited, the toxin kills the cell, its neighbors, and eliminates extracellular DNA via its DNase activity.

An example of an application for our project

Imagine a farmer that would like to know how much fertilizer is in his field, and optimize its use. We would provide him with cells carrying a nitrate biosensor (AgrEcoli), encapsulated in beads containing arabinose. He would spray the beads in his field, wait for 12h and then check if they are glowing in response to the nitrates contained in his soil.
We want to prevent the engineered organism or its DNA from being released and potentially transferred to a soil organism. For this reason once the arabinose is completely degraded inside the beads, the delay system would trigger the degradation of any DNA, followed by the collective death of our organisms due to the activation of toxic Colicins. Moreover, the semantic containment system would ensure that even if a synthetic gene is transferred to a natural organism, it would not be translated into a functional protein. Using our mechanism, the farmer would be able to use this device without endangering the environment by the release of synthetic genes.

Objectives

Our project aims to:

• Raise the issue of biosafety, and advocate the discerning use of biosafety circuits in future iGEM projects as a requirement
• Evaluate the risk of HGT in different SynBio applications
• Develop a new, improved containment system to expand the range of environments where GEOs can be used safely.

To do so, we:

• Engaged the general public and scientific community through debate
• Raised the question about how we can regulate this practices
• Compiled a parts page of safety circuits in the registry
• Relied on three levels of containment :
  1. Physical containment with alginate capsules
  2. Semantic containment using an amber suppressor system
  3. An improved killswitch featuring delayed population-level suicide through complete genome degradation.

We strived to make our system as robust against mutations as possible.