Team:Bielefeld-Germany/Project/Achievments

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Revision as of 23:35, 26 September 2012

Project Achievments

We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL). Results

We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. Results

We successfully produced purified and identified an active Bacillus pumilus DSM 27-Laccase (CotA named BPUL). Results

We successfully produced, purified an active Thermus thermophilus HB27-Laccase (tthL named TTHL). Results

We successfully produced an active Bacillus halodurans C-125e-Laccase (Lbh1 named BHAL). Results

We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol Results

Successful immobilization of Bacillus pumilus DSM 27 -Laccase (CotA named BPUL). Results

Successful immobilization of E. coli BL21-Laccase (CueO named ECOL). Results

Successful immobilization of Trametes versicolor-Laccase (named TVEL0). Results

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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

Contact Us

Universität Bielefeld
Universitätsstraße 25
D-33615 Bielefeld

info(at)igem-bielefeld.de

Navigate our Wiki

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-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced purified and identified an active ''E. coli'' BL21- Laccase (CueO named ECOL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#4 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced purified and identified an active ''E. coli'' BL21-Laccase (CueO named ECOL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#4 Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#11 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#11 Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''Bacillus pumilus'' DSM 27 -Laccase (CotA named BPUL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#3 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''Bacillus pumilus'' DSM 27-Laccase (CotA named BPUL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#3 Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced, purified  an active ''Thermus thermophilus'' HB27- Laccase (tthL named TTHL)  [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#5 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced, purified  an active ''Thermus thermophilus'' HB27-Laccase (tthL named TTHL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#5 Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced an active ''Bacillus halodurans'' C-125e -Laccase (Lbh1 named BHAL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced an active ''Bacillus halodurans'' C-125e-Laccase (Lbh1 named BHAL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We estabilished a method for degradation with our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of Bacillus pumilus DSM 27 -Laccase (CotA named BPUL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of ''Bacillus pumilus'' DSM 27 -Laccase (CotA named BPUL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of E. coli BL21- Laccase (CueO named ECOL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of ''E. coli'' BL21-Laccase (CueO named ECOL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of Trametes versicolor  - Laccase (named TVEL0) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of ''Trametes versicolor''-Laccase (named TVEL0). [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]