Revision as of 23:22, 26 September 2012

Module 6: Containment

Modelling

Aim For our containment module, we’ve created a model that shows how widespread the activity of nuclease would be given a certain colony. This would allow predictions to be made on how large a cell colony will be required to generate a halo diameter (indicating a DNA-free zone where the nuclease has digested the DNA in the agar) of desired size.

Methods

From the DNase agar test, the colony diameter (m), halo diameter (m), and absorbance at 600 OD were measured. From the absorbance values, we work out the cell concentration in cell per litres. This is done using a [http://www.genomics.agilent.com/CalculatorPopupWindow.aspx?CalID=7 third party calculator generated by Aligent Technologies].

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 == Modelling ==
-Our system uses a constitutive promoter for nuclease expression; however we wanted to find out what might be the results of using a different promoter so that nuclease would be expressed only at cell death.  Our gene network model shows the effects of different promoters on nuclease expression.
+'''Aim'''
+For our containment module, we’ve created a model that shows how widespread the activity of  nuclease would be given a certain colony.  This would allow predictions to be made on how large a cell colony will be required to generate a halo diameter (indicating a DNA-free zone where the nuclease has digested the DNA in the agar) of desired size.
+'''Methods'''
+From the DNase agar test, the colony diameter (m), halo diameter (m), and absorbance at 600 OD were measured. From the absorbance values, we work out the cell concentration in cell per litres. This is done using a [http://www.genomics.agilent.com/CalculatorPopupWindow.aspx?CalID=7 third party calculator generated by Aligent Technologies].
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Team:University College London/Module 6/Modelling

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Revision as of 23:22, 26 September 2012

Module 6: Containment

Modelling