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Team:University College London/HumanPractice/DIYbio/Workshops
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== Planning == | == Planning == | ||
| - | Prior to the wetlab workshops, we visited the | + | Prior to the wetlab workshops, we visited the Hackspace on several occasions to prepare for the wetlab sessions. This included a short introduction to synthetic biology and our project, a large health and safety discussion, and primer a design workshop. From our early interactions with the citizen scientists, we found that they have done many PCRs and gels before. Some of these have been successful. However, they have not attempted cloning before due to the lack of licensing and UK regulations. What they wanted from this collaboration is to visit our lab to see how we conduct the experiments in an academic institution and also to be able to perform all the steps in the cloning process. Hence, in the UCL workshop schedule we attempted to fit all the experiments in three days. |
'''Hackspace Workshop 29th-31st''' | '''Hackspace Workshop 29th-31st''' | ||
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<html><a href="https://static.igem.org/mediawiki/2012/b/b7/Ucl2012-Hackspace_Schedule_2930th_aug.pdf" target="_blank">Hackspace Wetlab Workshop schedule</a></html> | <html><a href="https://static.igem.org/mediawiki/2012/b/b7/Ucl2012-Hackspace_Schedule_2930th_aug.pdf" target="_blank">Hackspace Wetlab Workshop schedule</a></html> | ||
| - | In the Hackspace, we | + | In the Hackspace, we worked with the Biohackers on making competent ''E.coli'' (W3110) cells in the lab. This was an exciting experience, as we had to make a DIY incubator/shaker ourselves. We had help from many people in the Hackspace from sawing wood to circuit building. |
| + | {{:Team:University_College_London/fbphotosbytudelft|album=348430135241378|count=6}} | ||
'''UCL Workshops 3rd-5th September''' | '''UCL Workshops 3rd-5th September''' | ||
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<html><a href="https://static.igem.org/mediawiki/2012/2/24/Ucl2012-UCL_Laboratory_Schedule.pdf" target="_blank">Original UCL Wetlab Workshop schedule</a></html> | <html><a href="https://static.igem.org/mediawiki/2012/2/24/Ucl2012-UCL_Laboratory_Schedule.pdf" target="_blank">Original UCL Wetlab Workshop schedule</a></html> | ||
| - | In the beginning of September, we held three day | + | In the beginning of September, we held a three day workshop in UCL's teaching laboratory for the citizen scientists. We had an ambitious plan for the workshops, from genomic DNA extraction to ligation and transformation. This was a packed schedule. However, after day one, we quickly realised that it would be a much better learning experience for the Biohackers if the pace was slowed down. The amount of practical experience between these citizen scientists also varied. We allowed much longer time intervals between the practicals to answer the Biohackers questions and to show them proper techniques we use in the laboratory. |
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| + | Unfortunately, the first genomic DNA extraction was unsuccessful and some items delayed. However, the participants remarked how exciting and insightful they found the trouble-shooting that ensued. | ||
| + | {{:Team:University_College_London/fbphotosbytudelft|album=350135541737504|count=6}} | ||
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All protocols and results of the collaboration can be found on the London Hackspace wiki ([http://wiki.london.hackspace.org.uk/view/Public_Biobrick#Visualisation_and_interpretation 1]) | All protocols and results of the collaboration can be found on the London Hackspace wiki ([http://wiki.london.hackspace.org.uk/view/Public_Biobrick#Visualisation_and_interpretation 1]) | ||
Revision as of 22:21, 26 September 2012
Contents |
DIYbio
Overview | Concept | DIYbio | Workshops | Exhibition | Evaluation | Conclusion
Planning
Prior to the wetlab workshops, we visited the Hackspace on several occasions to prepare for the wetlab sessions. This included a short introduction to synthetic biology and our project, a large health and safety discussion, and primer a design workshop. From our early interactions with the citizen scientists, we found that they have done many PCRs and gels before. Some of these have been successful. However, they have not attempted cloning before due to the lack of licensing and UK regulations. What they wanted from this collaboration is to visit our lab to see how we conduct the experiments in an academic institution and also to be able to perform all the steps in the cloning process. Hence, in the UCL workshop schedule we attempted to fit all the experiments in three days.
Hackspace Workshop 29th-31st
Hackspace Wetlab Workshop schedule
In the Hackspace, we worked with the Biohackers on making competent E.coli (W3110) cells in the lab. This was an exciting experience, as we had to make a DIY incubator/shaker ourselves. We had help from many people in the Hackspace from sawing wood to circuit building.
UCL Workshops 3rd-5th September
Original UCL Wetlab Workshop schedule
In the beginning of September, we held a three day workshop in UCL's teaching laboratory for the citizen scientists. We had an ambitious plan for the workshops, from genomic DNA extraction to ligation and transformation. This was a packed schedule. However, after day one, we quickly realised that it would be a much better learning experience for the Biohackers if the pace was slowed down. The amount of practical experience between these citizen scientists also varied. We allowed much longer time intervals between the practicals to answer the Biohackers questions and to show them proper techniques we use in the laboratory.
Unfortunately, the first genomic DNA extraction was unsuccessful and some items delayed. However, the participants remarked how exciting and insightful they found the trouble-shooting that ensued.
All protocols and results of the collaboration can be found on the London Hackspace wiki ([http://wiki.london.hackspace.org.uk/view/Public_Biobrick#Visualisation_and_interpretation 1])
Result: Analytical Digest of the Public Biobrick
Safety and regulations
Legality of making competent cell protocol at London Hackspace
