Team:Goettingen/week18-3

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Latest revision as of 20:28, 26 September 2012


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Inoculation of LB-media for qrtPCR of different promoter constructs

First Test for Isolaton of RNA, Synthesis of cDNA and qrtPCR approach

Experiment:
RNA was isolated using Nuceleus Spin RNA II (Machery and Nagel).
cDNA synthesis was performed using Reverse Transcription Kit (Qiagen).
First approach of qrtPCR with different delutions of cDNA in H₂0
For a detailed protocol click here.
Observations & Results
cDNA which was 1:10 diluted in H₂0 showed the best results for qrtPCR approach of our promoter constructs.

@@ Line 39: / Line 39: @@
 - Tar receptor under the control of constitutive promoter J23112<br>
 - Tar receptor under the control of constitutive promoter J23113<br>
-- Tar receptor under the control of constitutive promoter J23114<br>
+- Tar receptor under the control of constitutive promoter J23114<br><br>
 For more details <a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts">click here</a>.<br>
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