Team:Paris Bettencourt/Achievements

Achievements :

• Construction and characterization of 2 biobricks :
  • [http://partsregistry.org/Part:BBa_K914000 K914000] : P_Lac-supD-T : tRNA amber suppressor
  • [http://partsregistry.org/Part:BBa_K914009 K914009] : P1003* Ser133->Amber Codon : kanamycin gene resistance with 1 amber mutation

The first part (supD) is well characterized and works well. For the second parts, it turns out that this mutation is quite leaky, although it works in lab conditions, one mutation is not enough if we want to release such parts in nature. Other reasons emphasize this observation, notably the weakness of being at one mutation to recover the protein functionality.

• Creation of a new category in the part registry : [http://partsregistry.org/Biosafety Semantic containment]. The aim of this category is to let people improving each part by adding for instance other amber mutations to existing part to increase the containment.

Achievements :

• Construction of 4 biobricks [Read more]:
  • [http://partsregistry.org/Part:BBa_K914003 K914003]: L-rhamnose-inducible promoter
  • [http://partsregistry.org/Part:BBa_K914005 K914005]: Meganuclease I-SceI controlled by pLac
  • [http://partsregistry.org/Part:BBa_K914007 K914007]: Meganuclease I-SceI controlled by pBad
  • [http://partsregistry.org/Part:BBa_K914008 K914008]: Meganuclease I-SceI controlled by pRha
• Demonstration that all 3 generators (K914005, K914007, K914008) work and express I-SceI meganuclease in cells. [Read more]

• Characterization of 2 biobricks from TUDelft [Read more]:
  • [http://partsregistry.org/Part:BBa_K175041 K175041]: p(LacI) controlled I-SceI homing endonuclease generator
  • [http://partsregistry.org/Part:BBa_K175027 K175027]: I-SceI restriction site
• Currently we are in process of L-rhamnose-inducible promoter (pRha) caracterisation. [Read more]