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Team:HokkaidoU Japan
From 2012.igem.org
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We have designed two modules to make E. coli implementing the function as bio-capsules that accumulate bio-plastic. | We have designed two modules to make E. coli implementing the function as bio-capsules that accumulate bio-plastic. | ||
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First module is to make bio-capsules formed by aggregation of E. coli through cell-cell interaction via adhesion molecule, Ag43, | First module is to make bio-capsules formed by aggregation of E. coli through cell-cell interaction via adhesion molecule, Ag43, | ||
located on the surface of outer membrane. The E. coli capsules can be easily collected by simple filtration without centrifuge steps. | located on the surface of outer membrane. The E. coli capsules can be easily collected by simple filtration without centrifuge steps. | ||
Removal of centrifuge steps largely contributes for reduction of time-consuming steps in industrial production of valuable material in E. coli. | Removal of centrifuge steps largely contributes for reduction of time-consuming steps in industrial production of valuable material in E. coli. | ||
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Second module is for producing bio-plastic (poly-3-hydroxybutyrate, P3HB). PHB is produced by microorganisms (like Ralstonia eutrophus). | Second module is for producing bio-plastic (poly-3-hydroxybutyrate, P3HB). PHB is produced by microorganisms (like Ralstonia eutrophus). | ||
The polymer is primarily a product of carbon assimilation from glucose and is important for microorganisms as an energy storage molecule. | The polymer is primarily a product of carbon assimilation from glucose and is important for microorganisms as an energy storage molecule. | ||
Revision as of 16:41, 26 September 2012
Bio-capsule E. coli
Spherical E. coli cluster that can accumulate macromolecules in it
We have designed two modules to make E. coli implementing the function as bio-capsules that accumulate bio-plastic.
First module is to make bio-capsules formed by aggregation of E. coli through cell-cell interaction via adhesion molecule, Ag43, located on the surface of outer membrane. The E. coli capsules can be easily collected by simple filtration without centrifuge steps. Removal of centrifuge steps largely contributes for reduction of time-consuming steps in industrial production of valuable material in E. coli.
Second module is for producing bio-plastic (poly-3-hydroxybutyrate, P3HB). PHB is produced by microorganisms (like Ralstonia eutrophus). The polymer is primarily a product of carbon assimilation from glucose and is important for microorganisms as an energy storage molecule. Development of a cost-effective method for manufacturing biodegradable plastic is one of important issue for making sustainable future society. We have optimized culture conditions for efficient production of E. coli bio-capsules and efficient production of P3HB (first success of bio-plastic production as iGEM team). Then we will try to extend the applicability of this system for producing other high-value added macromolecules in the capsule.
Biobrick Reviews
