Team:Goettingen/week14-1
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<li>Experiment: <br>The M9-minimal media was prepared as described in the material section. </li> | <li>Experiment: <br>The M9-minimal media was prepared as described in the material section. </li> | ||
<li>Observations & Results: <br>M9-media displayed brownish schlieren. Probably CaCl<sub>2</sub> and MgSO<sub>4</sub> should be sterilfiltrated and added to the autoclaved M9-medium afterwards. </li> | <li>Observations & Results: <br>M9-media displayed brownish schlieren. Probably CaCl<sub>2</sub> and MgSO<sub>4</sub> should be sterilfiltrated and added to the autoclaved M9-medium afterwards. </li> | ||
- | <br> | + | <br></ul> |
<b>V08_03_2: Motility assay with MG1655 and RP437</b><br> | <b>V08_03_2: Motility assay with MG1655 and RP437</b><br> | ||
- | <li>Experiment: <br>The over night cultures were not used to inoculate fresh LB. Instead, the cells were dropped directly onto 0,3% tryptone swimming plates and M9-plates for chemotaxis (as control also 2 LB-plates were used). 5 µl of each culture was dropped in the center of the tryptone plates and in 1 cm distance to the tryptone attractant on M9 plates, the drops were dried for 5min and put in an incubator at 37°C. The experiment was conducted in duplicates.</li> | + | <ul><li>Experiment: <br>The over night cultures were not used to inoculate fresh LB. Instead, the cells were dropped directly onto 0,3% tryptone swimming plates and M9-plates for chemotaxis (as control also 2 LB-plates were used). 5 µl of each culture was dropped in the center of the tryptone plates and in 1 cm distance to the tryptone attractant on M9 plates, the drops were dried for 5min and put in an incubator at 37°C. The experiment was conducted in duplicates.</li> |
<li>Observations & Results: <br>Minor growth and swimming was observed after 2 days at 37°C. One colony of MG1655 showed very strong swimming. Maybe lower incubation temperatures should be tested. </li> | <li>Observations & Results: <br>Minor growth and swimming was observed after 2 days at 37°C. One colony of MG1655 showed very strong swimming. Maybe lower incubation temperatures should be tested. </li> | ||
</ul> | </ul> |
Latest revision as of 14:26, 26 September 2012
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#1 Selection / Swimming - 14th WeekBack to overview
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