Team:Goettingen/week14-3

From 2012.igem.org

(Difference between revisions)
Line 49: Line 49:
</ul>
</ul>
<ul>
<ul>
-
<li>Observations & Results: <br>The correspodning agarose gel showed a band of the expected size.</li>
+
<li>Observations & Results: <br>The corresponding agarose gel showed a band of the expected size.</li>
</ul>
</ul>
<br>
<br>
Line 55: Line 55:
<ul>
<ul>
<li>Experiment: <br>The digestion was performed with a total volume of 300 µL according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">protocol of week 10</a>. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!</li>
<li>Experiment: <br>The digestion was performed with a total volume of 300 µL according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">protocol of week 10</a>. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!</li>
 +
</ul>
 +
<ul>
 +
<li>Observations & Results: <br>The corresponding agarose gel showed a band of the expected size.</li>
</ul>
</ul>
<br>
<br>
Line 62: Line 65:
</li>
</li>
</ul><br>
</ul><br>
 +
</td></tr>
 +
</table>
 +
<br>
 +
<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
 +
<tr bordercolor="black" valign="top">
 +
<td width="900" bordercolor="black" valign="top">
 +
<h2><b>V08_01 </b></h2><br>
 +
<b>V08_01_1 2<sup>nd</sup> round: Ethanol precipitation if ligation V07_31</b><br>
 +
<ul>
 +
<li>Experiment: <br>The ethanol precipitation was performed according to the established protocol form <a href="https://2012.igem.org/Team:Goettingen/week10-3">week 10</a>.</li>
 +
</ul>
 +
<ul>
 +
<li>Observations & Results: <br>The corresponding agarose gel showed a band of the expected size.</li>
 +
</ul>
 +
<br>
 +
<b>V08_01_2 2<sup>nd</sup> round: Transformation of electrocompetent cells with mutant plasmid mixture</b><br>
 +
<ul>
 +
<li>Experiment: <br>Electrocompetent cells were prepared according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The transformation was performed according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">protocol of week 10</a>.</li>
 +
</ul>
 +
<br>
</td></tr>
</td></tr>
</table>
</table>
Line 76: Line 99:
</html>
</html>
{{GoettingenFooter}}
{{GoettingenFooter}}
 +
b>V07_31_2 2
b>V07_31_2 2
b>V07_31_2 2
b>V07_31_2 2
b>V07_31_2 2

Revision as of 13:54, 26 September 2012

Deutsch  / English 

#3 Chemoreceptor Library - 14th Week

Back to overview

V07_30


V07_30_1 2nd round: Analysis of the transformation V07_27
  • Experiment:
    The plates and liquid culture were analyzed.
  • Observations & Results:
    Neither the liquid culture nor the plates exhibited bacterial growth.

V07_30_2 2nd round: Saturated mutagenesis PCR, 1000 µL
  • Experiment:
    The PCR was set up again, seeing as we lost our DNA material during the ethanol precipitation the previous week.


V07_31


V07_31_1 2nd round: PCR clean-up
  • Experiment:
    The PCR clean-up was performed according to the established protocol form week 10.
  • Observations & Results:
    The corresponding agarose gel showed a band of the expected size.

V07_31_2 2nd round: DpnI/BsaI digestion and purification
  • Experiment:
    The digestion was performed with a total volume of 300 µL according to protocol of week 10. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!
  • Observations & Results:
    The corresponding agarose gel showed a band of the expected size.

V07_31_3 1st round: Ligation
  • Experiment:
    The ligation was set up in a 100 µL batch according to protocol. Incubation over night at 16 °C.


V08_01


V08_01_1 2nd round: Ethanol precipitation if ligation V07_31
  • Experiment:
    The ethanol precipitation was performed according to the established protocol form week 10.
  • Observations & Results:
    The corresponding agarose gel showed a band of the expected size.

V08_01_2 2nd round: Transformation of electrocompetent cells with mutant plasmid mixture
  • Experiment:
    Electrocompetent cells were prepared according to protocol. The transformation was performed according to protocol of week 10.


Back to overview
↑ Back to top!
b>V07_31_2 2 b>V07_31_2 2 b>V07_31_2 2

Retrieved from "http://2012.igem.org/Team:Goettingen/week14-3"