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Team:BostonU/Notebook
From 2012.igem.org
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Revision as of 15:46, 27 June 2012
Notebook
May
Week 5:
- Orientation day
We met with our mentors and got an overview of the basics of synthetic biology, namely characterization, which will be the focus of our works during the summer. We also went through the “MoClo” technique, which we intend to introduce to iGEM.
- We did a survey of previous iGEM teams that got the Best Experimental Measurements award, then presented the findings to our mentors.
Week 1
- We performed PCR to amplify pMJSAF using the following MoClo forward and reverse primers.
- DVL0_AF with DVL0_BR (MF1)
- DVL0_BF with DVL0_CR (MF2)
- DVL0_CF with DVL0_DR (SJ1)
- DVL0_DF with DVL0_ER (SJ2)
- We performed Gel Electrophoresis to confirm that pMJSAF was amplified in PCR.
