Team:TMU-Tokyo/Notebook/Assay
From 2012.igem.org
(Difference between revisions)
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<p class="description"> | <p class="description"> | ||
<b>■Assay1(Device1)</b><Br> | <b>■Assay1(Device1)</b><Br> | ||
- | + | →<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_1_Protocol_and_Result/">Assay 1 Protocol and Result</A><Br><Br></p> | |
<p class="description"> | <p class="description"> | ||
<b>■Assay2(Device2)</b><Br> | <b>■Assay2(Device2)</b><Br> | ||
Quantitative Analysis of Formaldehyde by<Br> | Quantitative Analysis of Formaldehyde by<Br> | ||
- | + | →/">Assay 2 Protocol and Result<Br> | |
</p> | </p> | ||
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FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ | FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ | ||
at 340nm.<Br> | at 340nm.<Br> | ||
- | + | →/">Assay 3 Protocol and Result<Br> | |
</p> | </p> | ||
Revision as of 07:44, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Assay
■Assay1(Device1)
→Assay 1 Protocol and Result
■Assay2(Device2)
Quantitative Analysis of Formaldehyde by
→/">Assay 2 Protocol and Result
■Assay3(Device3)
FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺
at 340nm.
→/">Assay 3 Protocol and Result