"
Team:TMU-Tokyo/Notebook/Experiment/4th week (9. 3 - 9. 9)
From 2012.igem.org
(Difference between revisions)
| Line 62: | Line 62: | ||
<Br> | <Br> | ||
<Br> | <Br> | ||
| - | + | <b>■ 4th Sep<b/></p> | |
STEP 2: Construction of Device1 | STEP 2: Construction of Device1 | ||
Colony PCR of transformed E. coli (pfrm-frmR-GFP) | Colony PCR of transformed E. coli (pfrm-frmR-GFP) | ||
| Line 68: | Line 68: | ||
Failure: because of no bands | Failure: because of no bands | ||
| - | + | <b>■ 5th Sep<b/></p> | |
Ordered P.putida arrive | Ordered P.putida arrive | ||
STEP 2: Construction of Device1 | STEP 2: Construction of Device1 | ||
| Line 85: | Line 85: | ||
Success: 1 of 15 samples is appropriate band! | Success: 1 of 15 samples is appropriate band! | ||
| - | + | <b>■ 6th Sep<b/></p> | |
STEP 2: Construction of Device1 | STEP 2: Construction of Device1 | ||
Colony PCR of transformed E. coli (pfrm-frmR-GFP) | Colony PCR of transformed E. coli (pfrm-frmR-GFP) | ||
| Line 97: | Line 97: | ||
Failure: because of no bands | Failure: because of no bands | ||
| - | + | <b>■ 7th Sep<b/></p> | |
STEP 1: Construction of Device3 | STEP 1: Construction of Device3 | ||
PCR of insert (fdh4AB) | PCR of insert (fdh4AB) | ||
| Line 111: | Line 111: | ||
Cultivation of P.putida (FDH) on plate | Cultivation of P.putida (FDH) on plate | ||
| - | + | <b>■ 9th Sep<b/></p> | |
STEP 3: Construction of Device2 | STEP 3: Construction of Device2 | ||
PCR of insert (FDH) | PCR of insert (FDH) | ||
Revision as of 01:13, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
Experiment
■4th week (9. 3 - 9. 9)
■3rd September
STEP 1: Construction of Device3
PCR of insert (fdh4AB)
Electrophoresis
Failure: because of too dilute bands
PCR of insert (fdh4AB)
Electrophoresis
Failure: because of annealing temperature
PCR of insert (fdh4AB)
Electrophoresis
Success: We observed appropriate bands!
■ 4th Sep
