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Team:TMU-Tokyo/Notebook/Experiment/5th week (9.10 - 9.16)
From 2012.igem.org
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<b>■5th week (9.10 - 9.16)</b><Br></p> | <b>■5th week (9.10 - 9.16)</b><Br></p> | ||
<Br> | <Br> | ||
| + | <Br> | ||
| + | <p class="description3"> | ||
| + | <b>■10th Sep</b><br /><Br> | ||
| + | STEP 3: Construction of Device2<Br> | ||
| + | PCR of insert (FDH)<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Failure: because of a lot of smear<Br> | ||
| + | Ordered Device1 and Device2 plasmids arrive!<Br> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | <b>■11th Sep</b><br /><Br> | ||
| + | STEP 1: Construction of Device3<Br> | ||
| + | PCR of insert (fdh4AB)<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Failure: because of no bands<Br> | ||
| - | + | <b>■12th Sep</b><br /><Br> | |
| + | <Br> | ||
| + | STEP 1: Construction of Device3<Br> | ||
| + | Colony PCR of transformed E. coli (p-fdh4AB)<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Failure: because of transformation miss<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Failure: because of transformation miss<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Failure: because of transformation miss<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Failure: because of transformation miss<Br> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | <b>■13th Sep</b><br /><Br> | ||
| + | <Br> | ||
| + | STEP 1: Construction of Device3<Br> | ||
| + | PCR of insert (fdh4AB)<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Success: We observed appropriate bands but a lot of smear<Br> | ||
| + | Colony PCR of transformed E. coli (p-fdh4AB)<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Failure: because of no appropriate bands<Br> | ||
| + | <Br> | ||
| + | <Br> | ||
| + | <b>■14th Sep</b><br /> | ||
| + | New fdh4AB primers arrive<Br> | ||
| + | STEP 1: Construction of Device3<Br> | ||
| + | PCR of insert (fdh4AB)<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Failure: because of artificial mistake?<Br> | ||
| + | PCR of insert (fdh4AB)<Br> | ||
| + | Electrophoresis<Br> | ||
| + | Success: appropriate bands are clearer than before!<Br> | ||
| + | <Br> | ||
</p> | </p> | ||
</div> | </div> | ||
Revision as of 22:04, 25 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
Experiment
■5th week (9.10 - 9.16)
■10th Sep
STEP 3: Construction of Device2
PCR of insert (FDH)
Electrophoresis
Failure: because of a lot of smear
Ordered Device1 and Device2 plasmids arrive!
■11th Sep
STEP 1: Construction of Device3
PCR of insert (fdh4AB)
Electrophoresis
Failure: because of no bands
■12th Sep
STEP 1: Construction of Device3
Colony PCR of transformed E. coli (p-fdh4AB)
Electrophoresis
Failure: because of transformation miss
Electrophoresis
Failure: because of transformation miss
Electrophoresis
Failure: because of transformation miss
Electrophoresis
Failure: because of transformation miss
■13th Sep
STEP 1: Construction of Device3
PCR of insert (fdh4AB)
Electrophoresis
Success: We observed appropriate bands but a lot of smear
Colony PCR of transformed E. coli (p-fdh4AB)
Electrophoresis
Failure: because of no appropriate bands
■14th Sep
New fdh4AB primers arrive
STEP 1: Construction of Device3
PCR of insert (fdh4AB)
Electrophoresis
Failure: because of artificial mistake?
PCR of insert (fdh4AB)
Electrophoresis
Success: appropriate bands are clearer than before!
