"
Team:TMU-Tokyo/Notebook/Experiment/4th week (9. 3 - 9. 9)
From 2012.igem.org
| Line 47: | Line 47: | ||
<b>■4th week (9. 3 - 9. 9)</b><Br></p> | <b>■4th week (9. 3 - 9. 9)</b><Br></p> | ||
<Br> | <Br> | ||
| - | <b> | + | <p class="description3"> |
| + | <b>■3rd September<b/></p> | ||
| + | <p class="description3"> | ||
STEP 1: Construction of Device3<Br> | STEP 1: Construction of Device3<Br> | ||
PCR of insert (fdh4AB)<Br> | PCR of insert (fdh4AB)<Br> | ||
Revision as of 21:54, 25 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
Experiment
■4th week (9. 3 - 9. 9)
■3rd September
STEP 1: Construction of Device3
PCR of insert (fdh4AB)
Electrophoresis
Failure: because of too dilute bands
PCR of insert (fdh4AB)
Electrophoresis
Failure: because of annealing temperature
PCR of insert (fdh4AB)
Electrophoresis
Success: We observed appropriate bands!
Tuesday, 4th September
STEP 2: Construction of Device1
Colony PCR of transformed E. coli (pfrm-frmR-GFP)
Electrophoresis
Failure: because of no bands
Wednesday, 5th September
Ordered P.putida arrive
STEP 2: Construction of Device1
Electrophoresis
Failure: because of no bands
Colony PCR of transformed E. coli (pfrm-frmR-GFP)
Electrophoresis
Success: 2 of 15 samples are appropriate bands!
Electrophoresis
Failure: because of transformation miss
Electrophoresis
Failure: because of annealing temperature
Electrophoresis
Failure: because of annealing temperature
Electrophoresis
Success: 1 of 15 samples is appropriate band!
Thursday, 6th September
STEP 2: Construction of Device1
Colony PCR of transformed E. coli (pfrm-frmR-GFP)
Electrophoresis
Failure: because of transformation miss
Electrophoresis
Failure: because of transformation miss
STEP 1: Construction of Device3
Colony PCR of transformed E. coli (p-fdh4AB)
Electrophoresis
Failure: because of no bands
Friday, 7th September
STEP 1: Construction of Device3
PCR of insert (fdh4AB)
Electrophoresis
Success: We observed appropriate bands but a lot of smear.
Electrophoresis
Success: We observed appropriate bands!
Colony PCR of transformed E. coli (p-fdh4AB)
Electrophoresis
Failure: because of PCR condition or ligation miss
STEP 3: Construction of Device2
Preparation of culture plates for P.putida (FDH)
Cultivation of P.putida (FDH) on plate
Sunday, 9th September
STEP 3: Construction of Device2
PCR of insert (FDH)
Electrophoresis
Failure: because of no bands
