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| | ==Week 3 (05/14 - 05/20/12)== | | ==Week 3 (05/14 - 05/20/12)== |
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| - | === weekly seminar ===
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| - | * first lab service: Robert
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| - | * our GFP, which we wanted to use for the summer school for pupils, does not work.
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| - | * first competent cells have to be made: Julia S. and Robert.
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| - | * Decision to buy a commercial laccase to establish the analytics and the enzyme activity tests.
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| - | * our scientific exposé has to be translated into english: Malak
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| - | * Last planning for our waver sell.
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| - | * Julia S. is creating a vector for ''Pichia pastoris'' and is now searching for usable sequences.
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| - | * The [http://www.bmbf.de/en/index.php BMBF] invites all german iGEM teams to Berlin to attend at the Biotechnologie2020+ strategy process.
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| - |
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| - | ===Monday May 14th===
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| - | ===Tuesday May 15th===
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| - |
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| - | * '''Team Database''':
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| - | **Creating the logically design for the database. For example for our table 'Eppi' we opt for the following logic:
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| - | {|
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| - | |eppi ( || ID INT(11),
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| - | |-
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| - | | ||DataID INT(11),
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| - | |-
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| - | | ||Caption TEXT,
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| - | |-
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| - | | ||Content TEXT,
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| - | |-
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| - | | ||Experiment VARCHAR(255),
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| - | |-
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| - | | ||Where2 VARCHAR(255) ,
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| - | |-
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| - | | ||Location TEXT,
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| - | |-
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| - | | ||Coments TEXT,
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| - | |-
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| - | | ||Empty ENUM ('N', 'J'),
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| - | |-
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| - | | ||Create_User INT(11),
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| - | |-
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| - | | ||Creat_Date DATETIME,
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| - | |-
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| - | | ||Edit_Date DATETIME,
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| - | |-
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| - | | ||Delete ENUM ('N', 'J')
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| - | |-
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| - | | ||Delete_User INT(11),
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| - | |-
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| - | | ||Delete_Date DATETIME )
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| - | |}
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| - |
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| - | ===Wednesday May 16th===
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| - |
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| - | * '''Team Cloning of Bacterial Laccases''':
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| - | :*For cloning our laccases we need pSB1C3 backbone. Therefore we we transformed [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (pSB1C3 with RFP) in competent KRX cells.
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| - |
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| - | ===Thursday May 17th===
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| - | * '''Team Cloning of Bacterial Laccases''': Plasmid isolation of <partinfo>BBa_J04450</partinfo>.
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| - |
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| - | * '''Team Modeling''':
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| - | **Meeting Mrs. Lutter, a mathematics prof. of our course of studies and looking for our first model of a metabolic pathway, finding out, that we don't need such a complex model. Start thinking that we want and what we need.
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| - |
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| - | ===Friday May 18th===
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| - | * '''Team Activity Tests''': Our [http://www.sigmaaldrich.com/catalog/product/sigma/53739?lang=de®ion=DE ''T.versicolor'' laccase] and the [http://www.sigmaaldrich.com/catalog/product/sigma/53739?lang=de®ion=DE ABTS] arrived! We couldn´t wait to start, so we set up the stock solutions we will need, such as sodium acetat buffer (pH 5), 10 mM ABTS and deluted laccase.
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| - |
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| - | ===Saturday May 19th===
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| - | ===Sunday May 20th===
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| | {{Team:Bielefeld/Sponsoren}} | | {{Team:Bielefeld/Sponsoren}} |
"
