Revision as of 18:06, 25 September 2012

Module 4: Buoyancy

Characterisation

Starvation induction of E. coli transformed with this module will be done using minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3.0% and 5.0%). Quantification of the reporter output (GFP) from the samples taken will be measured using Tecan Zafire2 microplate detection system to quantify fluorescence intensity (485 nm for excitation, 520 nm for emission).

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 == Characterisation==
-In order to test the buoyancy of our cells, we will be determining their relative density. Cells will float or sink based on their density relative to the density of the solution surrounding them. Hence, by using different concentration of salt solutions, and observing the buoyancy of cells, a quantitative gauge of cell buoyancy can be determined.
+Starvation induction of ''E. coli'' transformed with this module will be done using minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3.0% and 5.0%). Quantification of the reporter output (GFP) from the samples taken will be measured using Tecan Zafire2 microplate detection system to quantify fluorescence intensity (485 nm for excitation, 520 nm for emission).
-We will also be directly visualising the gas vesicles in order to characterise them. This will be done by lysing the cells and extracting the gas vesicles, before preparing the samples for imaging by scanning electron microscopy. By analysing the gas vesicles ''ex vivo'', we expect to be able to obtain clear visualisation of the samples, allowing us to confirm gas vesicle production.
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Revision as of 18:06, 25 September 2012

Module 4: Buoyancy

Characterisation