Team:Goettingen/week2-1

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<h2><b>VX_Y </b></h2><br>
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<h2><b>V05_11 </b></h2><br>
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<b>Titel</b><br>
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<b>V05_11_1: Comparison of Yeast- and Beef-agar for Chemotaxis experiments</b><br>
<ul>
<ul>
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<li>Experiment: <br>hier text reinschreiben</li>
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<li>Experiment: <br>In this experiment it will be tested, if Yeast-agar (1%) or beef-agar (0.3%) is better for chemotaxis experiments. </li>
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<li>Observations & Results: <br>Booth strains show swarming on Yeast- (1%) and beef (0.3%) agar. Swimming in general very small. Maybe lower nutrition concentrations. </li>
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<br>
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<b>V05_11_2: Finding the right cell-concentrations for chemotaxis experiments</b><br>
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<ul>
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<li>Experiment: <br> We will test the cell concentrations between 10^6 and 10^9 cells/ml. We drop 10 µL on the agar plate. </li>
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<li>Observations & Results: <br> All cell-concentrations between 10^5 and 10^9 showed good swarming. All tested cell-concentrations are usable.</li>
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</ul>
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<br>
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<b>V05_11_3: Finding the right volumina for chemotaxis experiments</b><br>
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<ul>
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<li>Experiment: <br> Due to the problems with blured colonies we test different volumina for the colony-drop. Here are tested 1; 5 and 10 µL. </li>
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<li>Observations & Results: <br> The volumina 5 and 10 µL show swarming. The volume of 1 µL does not show swarming. 5 µL will be used for the further experiments</li>
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Latest revision as of 15:41, 25 September 2012

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#1 Selection / Swimming - 2nd Week

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V05_11


V05_11_1: Comparison of Yeast- and Beef-agar for Chemotaxis experiments
  • Experiment:
    In this experiment it will be tested, if Yeast-agar (1%) or beef-agar (0.3%) is better for chemotaxis experiments.
  • Observations & Results:
    Booth strains show swarming on Yeast- (1%) and beef (0.3%) agar. Swimming in general very small. Maybe lower nutrition concentrations.

V05_11_2: Finding the right cell-concentrations for chemotaxis experiments
  • Experiment:
    We will test the cell concentrations between 10^6 and 10^9 cells/ml. We drop 10 µL on the agar plate.
  • Observations & Results:
    All cell-concentrations between 10^5 and 10^9 showed good swarming. All tested cell-concentrations are usable.

V05_11_3: Finding the right volumina for chemotaxis experiments
  • Experiment:
    Due to the problems with blured colonies we test different volumina for the colony-drop. Here are tested 1; 5 and 10 µL.
  • Observations & Results:
    The volumina 5 and 10 µL show swarming. The volume of 1 µL does not show swarming. 5 µL will be used for the further experiments

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