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Team:Paris Bettencourt
From 2012.igem.org
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<p>During previous years’ competitions, many iGEM teams have developed projects that propose the application of Genetically Engineered Organisms (GEO) in natural environments. However, issues of biosafety continue to complicate and constrain the use of GEOs outside the lab. A primary concern is the Horizontal Gene Transfer (HGT) of synthetic genes to natural populations. Various strategies have been developed to address this problem, providing varying levels of containment. However, the substantial elimination of HGT risks remains difficult or perhaps impossible.<br> | <p>During previous years’ competitions, many iGEM teams have developed projects that propose the application of Genetically Engineered Organisms (GEO) in natural environments. However, issues of biosafety continue to complicate and constrain the use of GEOs outside the lab. A primary concern is the Horizontal Gene Transfer (HGT) of synthetic genes to natural populations. Various strategies have been developed to address this problem, providing varying levels of containment. However, the substantial elimination of HGT risks remains difficult or perhaps impossible.<br> | ||
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Revision as of 13:06, 25 September 2012
- Main
- Team
- Project
- Achievements
- Human Practice
- Safety
- Notebook
- Attributions
Overview
Delay system
Semantic containment
Restriction enzyme system
MAGE
Suicide system
Encapsulation
Synthetic import domain
Safety Questions
Safety AssessmentDuring previous years’ competitions, many iGEM teams have developed projects that propose the application of Genetically Engineered Organisms (GEO) in natural environments. However, issues of biosafety continue to complicate and constrain the use of GEOs outside the lab. A primary concern is the Horizontal Gene Transfer (HGT) of synthetic genes to natural populations. Various strategies have been developed to address this problem, providing varying levels of containment. However, the substantial elimination of HGT risks remains difficult or perhaps impossible.
Our project aims to:
- Raise the issue of biosafety, and advocate the discerning use of biosafety circuits in future iGEM projects as a requirement
- Evaluate the risk of HGT in different SynBio applications
- Develop a new, improved containment system to expand the range of environments where GEOs can be used safely.
To do so, we:
- Engaged the general public and scientific community through debate
- Raised the question about how we can regulate this practices
- Compiled a parts page of safety circuits in the registry
- Relied on three levels of containment :
- Physical containment with alginate capsules
- Semantic containment using an amber suppressor system
- An improved killswitch featuring delayed population-level suicide through complete genome degradation.
We will strive to make our system as robust against mutations as possible.
