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Team:Paris Bettencourt/Restriction Enzyme
From 2012.igem.org
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==Objectives== | ==Objectives== | ||
| - | + | # To find appropriate restriction enzymes which have to match the next properties: | |
| - | + | #* In the <i>E.Coli</i> genome there is no restriction sites of a choosen restriction enzyme; | |
| + | #* It has to have high specifity; | ||
| + | #* It have to works in wide range of different conditions (pH, T°C, etc) | ||
| + | # Choose very strong promoter to regulate restriction enzyme expression; | ||
| + | # To clone circuits with different combinations of choosen restriction enzymes and promoters. | ||
| + | # Mesure degradation efficiency for each circuit. | ||
| + | # Based on the best combinantion design self-disruption plasmid. | ||
==Design== | ==Design== | ||
Revision as of 22:28, 24 September 2012
- Main
- Team
- Project
- Achievements
- Human Practice
- Safety
- Notebook
- Attributions
Overview
Delay system
Semantic containment
Restriction enzyme system
MAGE
Suicide system
Encapsulation
Synthetic import domain
Safety Questions
Safety AssessmentTitle
Contents |
Overview
Our group was responsible for designing self-plasmid digestion system. This synthetic system allows to digest plasmids into linear parts of DNA which afterwards could be degraded by Colicin.
Objectives
- To find appropriate restriction enzymes which have to match the next properties:
- In the E.Coli genome there is no restriction sites of a choosen restriction enzyme;
- It has to have high specifity;
- It have to works in wide range of different conditions (pH, T°C, etc)
- Choose very strong promoter to regulate restriction enzyme expression;
- To clone circuits with different combinations of choosen restriction enzymes and promoters.
- Mesure degradation efficiency for each circuit.
- Based on the best combinantion design self-disruption plasmid.
Design
Present the design of your system, both in a written form, and a schematic one.
Experiments and results
Characterisation of X
Experimental setup
Describe the experiment
Results
Present your results
Testing of the system
Experimental setup
Describe the experiment
Results
Present your results
